Comparison of the Lactobacillus and Bifidobacterium Population in Fecal Microbiome of Celiac Disease Patients on Gluten-Free Diet With Healthy Subjects

Aims Celiac disease (CD) is a chronic autoimmune triggered by gluten and other environmental factors, such as intestinal microbiota in genetically predisposed persons. This study aimed to evaluate the composition of target gut population patients with CD compare it healthy individuals. Methods & Materials In this case-control study, Bifidobacterium Lactobacillus were evaluated fecal samples 20 celiac on gluten-free diet (GFD) individuals referred Disease Department, Tehran, Iran, from August 2019 February 2020. Microbial DNA extracted was specific primer pairs using real-time-polymerase chain reaction (PCR) technique. Statistical analysis performed IBM SPSS Statistics version 21. Findings The results demographic information participants regarding gender mean age well Marsh classification showed no statistically significant difference between two groups (P>0.05). comparison revealed that rate spp. significantly lower compared control group. Conclusion confirmed dysbiosis subjects. addition, changes microbiome may contribute pathogenesis CD.